Chemical-Enantiomeric Characterization and In-Vitro Biological Evaluation of the Essential Oils from <i>Elsholtzia strobilifera</i> (Benth.) Benth<i>.</i> and <i>E. blanda</i> (Benth.) Benth<i>.</i> from Nepal

Background: Essential oils (EOs) are one of the most important groups plant secondary metabolites responsible for their various biological activities. They also have wide applications as natural ingredients in many fields like food, cosmetic, and medicine industries. This study was conducted to determine chemical composition, enantiomeric distribution, in-vitro activities Elsholtzia strobilifera (Benth.) Benth. E. blanda essential Lamiaceae from Nepal. Methods: obtained hydro-distillation were analyzed by gas-chromatography mass-spectrometry (GC–MS) constituents distribution chiral terpenoids determined GC–MS. Microbroth dilution used investigate antimicrobial activity, while cytotoxic cell viability using a Cell Counting Kit assay. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging FRAP (ferric-reducing antioxidant power) assays evaluate Results: A total 55 compounds identified with pinocarvone (40.7%), β-pinene (10%) cis-pinocamphone (7.9%) predominant constituents. While, 41 EOs, including dihydrotagetone (49.1%), ( Z)-tagetone (15.6%) E)-tagetone (8%) major components; both dominated oxygenated monoterpenoids. separation analyses 12, 7, oil components performed, respectively, where (–)-β-caryophyllene (–)-germacrene D detected enantiomerically pure oils. EOs exhibited strong antifungal activity MIC value 156.3 µg/mL against Aspergillus niger, whereas, EO Staphylococcus epidermidis aureus showed inhibitory effects values µg/mL. very active cytotoxicity NIH-3T3 line IC 50 20.2 µg/mL, whereas displayed effective MCF-7 33.1 relatively good 188.8 ± 0.7 EC 348.0 0.1 FRAP, compared that EO. Conclusion: prominent emphasizing importance ingredients.